Virus:Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti- IV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse antiglutamine synthetase (Millipore, 1:500), rabbit and goat anti-GST (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibin (1:100, Abcam), rabbit anti-mouse Inhibin (1: 500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit antiNG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S) and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
Article Snippet: For oligodendrocyte progenitor and oligodendrocyte cells, the following primary antibodies were used: mouse anti-NG2 (1:25; eBioscience, San Diego, CA, USA), rabbit anti-PDGFRα (1:20; Abcam), rabbit anti-SOX10 (1:20; Abcam), mouse anti-OLIG1 (1:200; Millipore), mouse anti-A2B5 (1:50; Millipore), mouse anti-O4 (1:40; R&D Systems, Minneapolis, MN, USA), mouse anti-O1 (1:40; R&D Systems), and rat anti-Myelin Basic Protein (1:40; Abcam).
Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti-βIV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse anti-glutamine synthetase (Millipore, 1:500), rabbit and goat anti-GSTπ (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibinα (1:100, Abcam), rabbit anti-mouse Inhibinα (1:500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit anti-NG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD PharmigenTM 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S), and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596 S) and chicken anti-GFP (1:100, Abcam ab13970).
shRNA:Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti- IV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse antiglutamine synthetase (Millipore, 1:500), rabbit and goat anti-GST (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibin (1:100, Abcam), rabbit anti-mouse Inhibin (1: 500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit antiNG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S) and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
Article Snippet: For oligodendrocyte progenitor and oligodendrocyte cells, the following primary antibodies were used: mouse anti-NG2 (1:25; eBioscience, San Diego, CA, USA), rabbit anti-PDGFRα (1:20; Abcam), rabbit anti-SOX10 (1:20; Abcam), mouse anti-OLIG1 (1:200; Millipore), mouse anti-A2B5 (1:50; Millipore), mouse anti-O4 (1:40; R&D Systems, Minneapolis, MN, USA), mouse anti-O1 (1:40; R&D Systems), and rat anti-Myelin Basic Protein (1:40; Abcam).
Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti-βIV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse anti-glutamine synthetase (Millipore, 1:500), rabbit and goat anti-GSTπ (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibinα (1:100, Abcam), rabbit anti-mouse Inhibinα (1:500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit anti-NG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD PharmigenTM 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S), and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596 S) and chicken anti-GFP (1:100, Abcam ab13970).
Recombinant:Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti- IV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse antiglutamine synthetase (Millipore, 1:500), rabbit and goat anti-GST (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibin (1:100, Abcam), rabbit anti-mouse Inhibin (1: 500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit antiNG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S) and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
Article Snippet: For oligodendrocyte progenitor and oligodendrocyte cells, the following primary antibodies were used: mouse anti-NG2 (1:25; eBioscience, San Diego, CA, USA), rabbit anti-PDGFRα (1:20; Abcam), rabbit anti-SOX10 (1:20; Abcam), mouse anti-OLIG1 (1:200; Millipore), mouse anti-A2B5 (1:50; Millipore), mouse anti-O4 (1:40; R&D Systems, Minneapolis, MN, USA), mouse anti-O1 (1:40; R&D Systems), and rat anti-Myelin Basic Protein (1:40; Abcam).
Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti-βIV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse anti-glutamine synthetase (Millipore, 1:500), rabbit and goat anti-GSTπ (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibinα (1:100, Abcam), rabbit anti-mouse Inhibinα (1:500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit anti-NG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD PharmigenTM 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S), and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596 S) and chicken anti-GFP (1:100, Abcam ab13970).
Gene Expression:Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti- IV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse antiglutamine synthetase (Millipore, 1:500), rabbit and goat anti-GST (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibin (1:100, Abcam), rabbit anti-mouse Inhibin (1: 500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit antiNG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S) and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
Article Snippet: For oligodendrocyte progenitor and oligodendrocyte cells, the following primary antibodies were used: mouse anti-NG2 (1:25; eBioscience, San Diego, CA, USA), rabbit anti-PDGFRα (1:20; Abcam), rabbit anti-SOX10 (1:20; Abcam), mouse anti-OLIG1 (1:200; Millipore), mouse anti-A2B5 (1:50; Millipore), mouse anti-O4 (1:40; R&D Systems, Minneapolis, MN, USA), mouse anti-O1 (1:40; R&D Systems), and rat anti-Myelin Basic Protein (1:40; Abcam).
Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti-βIV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse anti-glutamine synthetase (Millipore, 1:500), rabbit and goat anti-GSTπ (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibinα (1:100, Abcam), rabbit anti-mouse Inhibinα (1:500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit anti-NG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD PharmigenTM 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S), and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596 S) and chicken anti-GFP (1:100, Abcam ab13970).
Fractionation:Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti- IV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse antiglutamine synthetase (Millipore, 1:500), rabbit and goat anti-GST (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibin (1:100, Abcam), rabbit anti-mouse Inhibin (1: 500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit antiNG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S) and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
Article Snippet: For oligodendrocyte progenitor and oligodendrocyte cells, the following primary antibodies were used: mouse anti-NG2 (1:25; eBioscience, San Diego, CA, USA), rabbit anti-PDGFRα (1:20; Abcam), rabbit anti-SOX10 (1:20; Abcam), mouse anti-OLIG1 (1:200; Millipore), mouse anti-A2B5 (1:50; Millipore), mouse anti-O4 (1:40; R&D Systems, Minneapolis, MN, USA), mouse anti-O1 (1:40; R&D Systems), and rat anti-Myelin Basic Protein (1:40; Abcam).
Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti-βIV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse anti-glutamine synthetase (Millipore, 1:500), rabbit and goat anti-GSTπ (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibinα (1:100, Abcam), rabbit anti-mouse Inhibinα (1:500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit anti-NG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD PharmigenTM 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S), and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596 S) and chicken anti-GFP (1:100, Abcam ab13970).
Luciferase:Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti- IV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse antiglutamine synthetase (Millipore, 1:500), rabbit and goat anti-GST (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibin (1:100, Abcam), rabbit anti-mouse Inhibin (1: 500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit antiNG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S) and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
Article Snippet: For oligodendrocyte progenitor and oligodendrocyte cells, the following primary antibodies were used: mouse anti-NG2 (1:25; eBioscience, San Diego, CA, USA), rabbit anti-PDGFRα (1:20; Abcam), rabbit anti-SOX10 (1:20; Abcam), mouse anti-OLIG1 (1:200; Millipore), mouse anti-A2B5 (1:50; Millipore), mouse anti-O4 (1:40; R&D Systems, Minneapolis, MN, USA), mouse anti-O1 (1:40; R&D Systems), and rat anti-Myelin Basic Protein (1:40; Abcam).
Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti-βIV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse anti-glutamine synthetase (Millipore, 1:500), rabbit and goat anti-GSTπ (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibinα (1:100, Abcam), rabbit anti-mouse Inhibinα (1:500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit anti-NG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD PharmigenTM 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S), and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596 S) and chicken anti-GFP (1:100, Abcam ab13970).
Software:Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti- IV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse antiglutamine synthetase (Millipore, 1:500), rabbit and goat anti-GST (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibin (1:100, Abcam), rabbit anti-mouse Inhibin (1: 500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit antiNG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S) and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
Article Snippet: For oligodendrocyte progenitor and oligodendrocyte cells, the following primary antibodies were used: mouse anti-NG2 (1:25; eBioscience, San Diego, CA, USA), rabbit anti-PDGFRα (1:20; Abcam), rabbit anti-SOX10 (1:20; Abcam), mouse anti-OLIG1 (1:200; Millipore), mouse anti-A2B5 (1:50; Millipore), mouse anti-O4 (1:40; R&D Systems, Minneapolis, MN, USA), mouse anti-O1 (1:40; R&D Systems), and rat anti-Myelin Basic Protein (1:40; Abcam).
Article Title: White Matter Stroke Induces a Unique Oligo-Astrocyte Niche That Inhibits Recovery
Article Snippet: Primary antibodies were as follows (in alphabetical order): rabbit anti-6xHis antibody (Abcam, 1:1000), rabbit anti-Activin Receptor type IIA (ActRIIA, Abcam, 1:200), rabbit anti-Aldh1 (1:1000, Abcam), mouse anti-βIV-spectrin (1:200, Neuromab), rabbit anti-Caspr (1:500, Abcam), mouse anti-CC1 (1:200, Abcam), rabbit anti CC1 (1:100, EMD Millipore), chicken anti-GFP (1:1000, Abcam), goat anti-GFP (1:5000, gift from Dr. Nathaniel Heintz, Rockefeller University), mouse anti-glutamine synthetase (Millipore, 1:500), rabbit and goat anti-GSTπ (both 1:500, Abcam), rabbit anti-Iba1 (1:1000, Wako Chemicals), mouse anti-human Inhibinα (1:100, Abcam), rabbit anti-mouse Inhibinα (1:500, Abcam), rabbit anti-Matrilin-2 (1:200, Abcam; and 1:100, Santa Cruz Biotechnology), chicken anti-mCherry (Novus Biologicals, 1:500), rat anti-myelin basic protein (MBP, 1:1000, EMD Millipore), rabbit anti-NG2 (1:200, EMD Millipore), mouse anti-Olig1 (EMD Millipore), rat anti-Olig1 (gift from Dr. Bennett Novitch, UCLA), and rabbit anti-Olig2 (1:500, EMD Millipore).
Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD PharmigenTM 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596S), and chicken anti-GFP (1:100, Abcam ab13970).
Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), rabbit anti-ABCA1 (1:100, Novus Biologicals NB400-105), mouse anti-Sox10 (1:100, Sigma-Aldrich AMAB91297), mouse anti-Olig1 (1:1000, EMD Millipore MAB5540), rabbit anti-active Caspase-3 (1:500, BD Pharmingen 559565), mouse anti-CNPase (1:2000, Sigma-Aldrich AMAB91072), mouse anti-MASH1 (1:100, BD Bioscience 556604), rabbit anti-NQO1 (1:100, Abcam ab2346), rat anti-NRF2 (1:500, Cell Signalling 14596 S) and chicken anti-GFP (1:100, Abcam ab13970).
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